Biological treatment of hospital and textile wastewaters using laccase-rich crude extracts from Trametes villosa

Maire Oliveira da Silva; Marcus Vinicius de Liz; Wanessa Algarte Ramsdorf; Eduardo Malosti Barbosa; Leticia Harumi Tesuka; Nayara Pereira Lima; Ludmila da Conceição Ferreira; Charles Windson Isidoro Haminiuk; Giselle Maria Maciel

doi:10.5327/z2176-94782875

Authors

Maire Oliveira da Silva Universidade Tecnológica Federal do Paraná (UTFPR) - Brazil https://orcid.org/0009-0000-0890-1215
Marcus Vinicius de Liz Universidade Tecnológica Federal do Paraná (UTFPR) - Brazil https://orcid.org/0000-0003-1370-3365
Wanessa Algarte Ramsdorf Universidade Tecnológica Federal do Paraná (UTFPR) - Brazil https://orcid.org/0000-0002-8341-1408
Eduardo Malosti Barbosa Universidade Tecnológica Federal do Paraná (UTFPR) - Brazil https://orcid.org/0009-0007-3623-668X
Leticia Harumi Tesuka Universidade Tecnológica Federal do Paraná (UTFPR) - Brazil https://orcid.org/0009-0009-6551-8832
Nayara Pereira Lima Universidade Federal do Paraná (UFPR) - Brazil https://orcid.org/0000-0003-0945-5856
Ludmila da Conceição Ferreira Universidade Federal do Paraná (UFPR) - Brazil https://orcid.org/0000-0002-2925-0818
Charles Windson Isidoro Haminiuk Universidade Tecnológica Federal do Paraná (UTFPR) - Brazil https://orcid.org/0000-0002-1432-9427
Giselle Maria Maciel Universidade Tecnológica Federal do Paraná (UTFPR) - Brazil https://orcid.org/0000-0003-3343-3059

DOI:

https://doi.org/10.5327/z2176-94782875

Keywords:

emerging contaminants; oxidoreductases; white-rot fungi; antibiotics; effluents, laccase.

Abstract

Hospital and textile wastewater represent distinct sources of environmental contamination, characterized by complex mixtures of micropollutants, including pharmaceuticals, dyes, and phenolic compounds. Widespread use, inadequate disposal, and the limited efficiency of conventional wastewater treatment processes have led to the accumulation of these micropollutants in aquatic environments. The structural complexity of these effluents poses a significant challenge to standardizing effective wastewater treatment methodologies. Oxidative enzymes, such as laccases, have emerged as potential tools for effluent remediation, improving water quality and reducing contamination. This study evaluated the application of an enzyme extract containing laccases produced by Trametes villosa for the treatment of hospital and textile wastewater. Analysis of hospital effluent samples by liquid chromatography coupled to mass spectrometry showed a significant reduction in antibiotic concentrations (97% for pyrazinamide, 74% for rifampicin, 66% for sulfamethoxazole, and 64% for trimethoprim) after enzymatic treatment compared with the control. Phenolic compounds were reduced by 64% in hospital effluents and by 98% in textile effluents. The chemical oxygen demand increased after enzymatic treatment in the hospital sample, while only a slight reduction was observed in the textile sample. The textile effluent treated with 1,000 U L-1 of laccase showed an 18% decrease in the visible spectral region and a 23% reduction in the maximum absorbance peak. In the phytotoxicity test with Lactuca sativa, effluents reduced seed growth, and enzymatic treatment reduced their toxicity. Thus, enzymatic treatment showed promising results as a sustainable strategy for treating hospital and textile wastewater.